The B cell receptor (BCR) is essential for survival of mature B cells and its specificity dictates the cells’ fate from early development through final differentiation into an effector or memory cell.  For example, immature B cells bearing a self-reactive BCR may be eliminated in the bone marrow (check-point I) or spleen (check point II).  Escape at either checkpoint could result in autoimmune disease.   A long-standing interest of the Carroll lab is how B cells encounter antigens, both self- and non-self, and how the local environment influences the outcome.

Antigens are“presented”to B cells in large part by follicular dendritic cells (FDC) via a pathway dependent on complement C3 and receptors CD21 and CD35 (see Figure 1).  Antigen recognition by antibody or pattern recognition receptors such as the C-type lectins can activate complement resulting in covalent attachment of the third component (C3) to the antigen.  Activated C3 provides a molecular tag that identifies the antigen as foreign.  C3-tagged antigens are transported to FDC via cells bearing complement receptors.  By a mechanism that is not clear, foreign antigens are retained in their native form and made available to cognate B cells over extensive periods.  Whether a similar pathway is involved in “presenting” self-antigen to immature B cells is not known. 

In summary, understanding how foreign antigens are made available to B cells at varying stages in their differentiation would be useful in the design of vaccines and programming of an optimal humoral response to specific pathogens.  Similarly, disrupting B cell access to self-antigen would be desirable in tuning-down the humoral response in autoimmunity.  For a description of related projects on-going in the Carroll Lab see the Multi-media page link:

                                                                                                                             Figure 1

Figure 1.  The complement system participates at various stages in B cell differentiation in humoral immunity. Complement receptors CD21 and CD35 play an important role in at least three stages of B cell differentiation: (1) co-ligation of C3d-antigen with BCR lowers threshold of B cell activation.; (2) activated B cells enter a germinal center; (3) clonal selection of GC B cells is dependent on contact with antigen and C3d-ligand.

 

Michael Carroll received his Ph.D. in Immunology from the UT Southwestern Medical School (Dallas, TX) under the direction of Dr. J. Donald Capra in 1980; subsequently he trained with Dr. Rodney R. Porter in the Biochemistry Department, Oxford U (Oxford UK). In 1985, he was appointed an Assistant Professor in Pediatrics and the Department of Biological Chemistry at the Children’s Hospital/Harvard Medical School. He was promoted in 1998 to the rank of Professor of Pediatrics, Harvard Medical School and Senior Investigator, Boston Children's Hospital, Program in Cellular and Molecular Medicine.

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