Instrumentation/Equipment

SYSTEM 1 -Confocal-

Olympus Fluoview FV1000 confocal system

Configuration: Inverted, with Olympus IX 81 microscope
Lasers lines: 405, 457, 488, 515, 559, 635 nms
Detectors (PMTs): 4 fluorescence +1 transmission; DIC and spectral imaging capability
Location: 3 Blackfan Circle, 3rd/Fl., Rm3123

 

 

SYSTEM 2 -Multiphoton-

Olympus FV1200 MPE multiphoton system

Configuration: Upright, with Olympus BX 61 WI microscope; customized for intravital imaging
Lasers: Spectra Physics MaiTai HP DeepSee, tuning range = 690-1020 nm
Detectors: 4 non-descanned (2 GaAsP & 2 regular PMTs)
Location: 200 Longwood Ave., WAB Rm274A

 

 

SYSTEM 3 -Spinning Disk Confocal-

Marianas SDC system, with Yokogawa CSU22 Spinning Disk

Configuration: inverted, with Ziess Axiovert 200M microscope, full enclosure environmental chamber
Lasers: 491, 561 and 642nm
Detectors: EM-CCD for confocal, wide-field CCD for wide-field
Location: 200 Longwood Ave., WAB Rm175

 

 

COMPUTER WORKSTATIONS WITH SPECIALIZED IMAGE PROCESSING SOFTWARE

2 Macs and 2 PCs
Software examples: AutoDeblur, Confocal Assistant, Image J, Imaris, Lasersharp, SlideBook, Volocity, VoxBlast