Instrumentation/Equipment

Equipment

IVIS SPECTRUM (molecular imaging in vivo)

 The IVIS SPECTRUM is one of the most versatile and advanced molecular imaging systems. The primary use of instrumentation is non-invasively monitor bioluminescence and fluorescence in small animals.  It has an incredibly sensitive camera combined with high energy trans- and epi- illumination that allows for bioluminescence and fluorescence detection. The imaging of up to 4 small animals, tissue samples and cell lines in the plates is possible. The system has 3D software and spectral unmixing capabilities. Location: FICR satellite facility at the NRB0044 room at the HCCM (24/7 access). The system also includes Living Image software allowing for advanced quantification in 3 dimensions. The instrument is available for not-supervised acquisition after training. This instrument is widely used in pre-clinical studies. For access to Calendar, please sign with FICR at iLABS (hourly fee for access to instrument is $60 per hour). 

FACSARIA cell sorters

We currently have three cell sorters: two 4- and one  5-laser FACSARIA II systems systems (A: 488, 640, 405/375;B: 488, 561, 640, 405;C: 355, 405, 488, 640, 594; FCS-PMT module and unique optics for microobjects). Instruments at 263A-WAB (FACSAria A) and 231-Armenise Special Order FACSAria C) rooms are open to users (7/24) after comprehensive training. The cell sorter B located in Baker Biohood (room 237) and requires assistance of trained staff (contact Kenneth Ketman at kenneth.ketman@childrens.harvard.edu). New sorter SONY SH800 (sorter D) will become available soon for independent sorting.

FACSCanto analyzers

We have 2 FACSCanto (488, 640, 405 lasers) at WAB 1 st floor, room 146 and CLSB room 3034 satellite locations (24/7 access). The FACSCanto-CLSB is getting BD Biosciences 96-well module. Two FACSCaliburs located in investigators laboratories are going to retire.

Imaging cytomer Imagestream X Mark II

 The  Imagestream X Mark II (lasers: 405 nm; 488 nm, 561 nm;640 nm; darkfield laser),equipped with Multimag option (40x; 60x objectives; 0.75 and 0.9 numerical aperture) and twelve image channels configuration. The Imagestream X Mark II is capable to acquire cells in 10 fluorescent channels, brightfield and darkfield channels, with a speed up to 5,000 events/sec.