Flow and Imaging CytometryResource
Program in Cellular and Molecular Medicine at Boston Children's Hospital
200 Longwood Avenue
Armenise Building D-239
Boston, MA 02115
Natasha S. Barteneva, MD, PhD,
Director, Flow & Imaging Cytometry Resource, PCMM/BCH
Lecturer on Pediatrics, Harvard Medical School
IVIS SPECTRUM (molecular imaging in vivo)
An IVIS SPECTRUM was recently acquired with the NIH Shared Instrumentation grant (scientific team led by Judy Lieberman). The IVIS SPECTRUM is one of the most versatile and advanced molecular imaging systems. It has an incredibly sensitive camera combined with high energy trans- and epi- illumination that allows for bioluminescence and fluorescence detection. The system has 3D software and spectral unmixing capabilities. Location: FICR satellite facility at the NRB0044 room at the HCCM.
We currently have two 4- and one 5-laser FACSARIA II systems systems (A: 488, 640, 405/375;B: 488, 561, 640, 405;C: 355, 405, 488, 640, 594; FCS-PMT module and unique optics for microobjects). Instruments at 263A-WAB (FACSAria A) and 231-Armenise Special Order FACSAria C) rooms are open to users (7/24) after comprehensive training. The cell sorter B located in Baker Biohood at BL2+-module and requires assistance of trained staff ( contact Kenneth Ketman at firstname.lastname@example.org).
We have 2 FACSCanto (488, 640, 405 lasers) at WAB 1 st floor and CLSB room 3034 satellite locations (24/7 access). Two FACSCaliburs (WAB and CLSB locations) are going to be refurbished soon.
The Imagestream 100 instrument acquired in 2007 and sponsored by NIH Shared Instrumentation grant (PI: Judy Lieberman) located in the room #237 Armenise Building in BL2+ module.
The fluorescence detection bands are fixed (470-500 nm; 400-470 nm; 500-560 nm; 560-595 nm; 595-660 nm; 660-735 nm). The system is compatible with a wide variety of fluorochromes and fluorescent proteins used in flow cytometry and microscopy.