Flow and Imaging Cytometry Resource
Flow and Imaging CytometryResource
Program in Cellular and Molecular Medicine at Boston Children's Hospital
200 Longwood Avenue
Armenise Building D-239
Boston, MA 02115
Natasha S. Barteneva, MD, PhD,
Director, Flow & Imaging Cytometry Resource, PCMM/BCH
Lecturer on Pediatrics, Harvard Medical School
The Flow and Imaging Cytometry Resource provides 1) fluorescence-activated cell sorting; 2) in vivo molecular imaging with IVIS SPECTRUM; 3) imaging cytometry services and extensive training to all investigator labs from the Program in Cellular and Molecular Medicine, the Boston Children's Hospital and HMS scientific community on case-by-case basis. We are available for grant-funded collaborative research community in and outside BCH and HMS.
Our recent publications:
NS Barteneva*, Ponomarev E, Tsytsykova A, Armant M, Vorobjev IA. Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting. JHC 62(4): 265-275, (2014).
B Yoo, A Kawishwar,SK Gosh, N Barteneva, MV Yigit, A Moore, Z Medarova. Detection of miRNA expression in intact cells using activatable sensor oligonucleotides. Chem Biol, Epub Jan 14 (2014).
NS Barteneva*, K Ketman, E Fasler-Kan, D Potashnikova, IA Vorobjev. Cell sorting in cancer research-diminishing degree of cell heterogeneity. BBA Rev on Cancer Res, 1836: 105-122 (2013).
SK Ghosh, MV Yigit, M Uchida, AW Ross, N Barteneva, A Moore, Z Medarova. Sequence-dependent combination therapy with doxorubicin and a survivin-specific small interfering TNA nanodrug demonstrates efficacy in models of adenocarcinoma. Inter J Cancer, EPub October (2013).
PY Mantel, AN Hoang, I Goldowitz, D Potashnikova, B Hamza, I Vorobjev, I Ghiran, I, M Toner, D Irimia, A Ivanov, N Barteneva, M Marti. Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host Microbe 13:521-534 (2013).
NS Barteneva*, N Maltsev, IA Vorobjev. Microvesicles and intercellular communication in the context of parasitism. Frontiers Cell Inf Microb 3: 49 (2013).
NS Barteneva*, E Fasler-Kan, M Bernimoulin, JNH Stern, L Duckett, ED Ponomarev, IA Vorobjev. Circulating microparticles: square the cycle. BMC Cell Biology 14: 23 (2013).
I Sotnikov, T Veremeyko, S-C Starossom, N Barteneva, HL Weiner, ED Ponomarev. Platelets recognize brain-specific glycolipid structures, respond to neurovascular damage and promote neuroinflammation. PLoS One, 8: e58979(2013).
NS Barteneva*, E Fasler-Kan, IA Vorobjev. Imaging flow cytometry: coping with heterogeneity in biological systems. JHC 60: 723-733 (2012).
IA Vorobjev, K Buchholz, P Prabhat, K Ketman, E Egan, M Marti, M Duraisingh, NS Barteneva*. Optimization of the flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters. Malaria J, 11: 312 (2012).
ED Ponomarev, T Veremeyko, N Barteneva, AM Krichevsky, HL Weiner. MicroRNA-124 promotes microglia quiescence and suppresses EAE by deactivating macrophages via the C/EBP-alpha-PU.1 pathway. Nat Med 17: 64-70 (2011).
EI Kovalenko, S Ranjbar, LD Jasenosky, AE Goldfeld, IA Vorobjev, NS Barteneva*. HaloTag-based technology in flow and laser scanning cytometry analysis of live and fixed cells. BMC Res Notes 4: 340 (2011).
ED Ponomarev, T Veremeyko, NS Barteneva*. Vizualization and quantitation of the expression of microRNAs and their target genes in neuroblastoma single cells using imaging cytometry. BMC Res Notes 4:517(2011).