The Flow and Imaging Cytometry Resource provides access to instrumentation for 1) fluorescence-activated cell sorting; 2) in vivo molecular imaging with IVIS SPECTRUM; 3) imaging flow cytometry and extensive training to all investigator labs from the Program in Cellular and Molecular Medicine, the Boston Children's Hospital and HMS scientific community on case-by-case basis. We are available for grant-funded collaborative research community in and outside BCH and HMS. Fee-for-services and training inquires should be directed to Flow and Imaging Cytometry Resource Lab Manager Kenneth Ketman (kenneth.ketman@childrens.harvard.edu).

 

Our Books: Imaging Flow Cytometry: Methods and Protocols. Editors: Natasha S Barteneva, Ivan A Vorobjev, Humana press, 2016, 295 pp. 

Selected Publications on Imaging Flow Cytometry and laser scanning cytometry:

Dashkova V, Malashenkov D, Poulton N, Vorobjev I, Barteneva NS*. Imaging flow cytometry for phytoplankton analysis. Methods 112: 105-123 (2017)

Vorobjev IA, Barteneva NS. Multi-parametric imaging of cell heterogeneity in apoptosis analysis. Methods 112: 105-123 (2017)

Wheeler LA, Trifonova RT, Vrbanac V, Barteneva NS, Liu X, Bollman B, Onofrey L, Mulik S, Ranjbar S, Luster AD, Tager AM, Lieberman J. TREX1 knockdown induces as interferon response to HIV that delays viral infection in humanized mice. Cell Reports 15: 1715-1727 (2016)

Haridas V, Ranjbar S, Vorobjev I, Goldfeld AE, Barteneva NS. Imaging flow cytometry analysis of intracellular pathogens. Methods 112: 91-104 (2017)

Selected Publications on multi-parametric cell sorting:

Gaudin R, Barteneva NS. Sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles. Nature Comm 6: 6022. doi: 10.1038/ncomms7022 (2015).

Barteneva NS*, Ponomarev ED, Tsytsykova A, Armant M, Vorobjev IA. Mitochondrial staining allows robust elimination of apoptotic and damaged cells. JHC 62(4): 265-275 (2014).

Barteneva NS*, Ketman K, Fasler-Kan E, Potashnikova D, Vorobjev IA. Cell sorting in cancer research - diminishing degree of cell heterogeneity. BBA Reviews in Cancer Research 1836(1): 105-122 (2013).

Vorobjev IA, Buchholz K, Prabhat P, Ketman K, Egan ES, Marti M, Duraisingh MT, Barteneva NS.Optimization of flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters. Malaria Journal 11:312. doi: 10.1186/1475-2875-11-312 (2012).

Selected Publications on extracellular vesicles:

PY Mantel, AN Hoang, I Goldowitz, D Potashnikova, B Hamza, I Vorobjev, I Ghiran, I, M Toner, D Irimia, A Ivanov, N Barteneva, M Marti. Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host Microbe 13:521-534 (2013).

NS Barteneva*, N Maltsev, IA Vorobjev. Microvesicles and intercellular communication in the context of parasitism. Frontiers Cell Inf Microb 3: 49 (2013).

NS Barteneva*, E Fasler-Kan, M Bernimoulin, JNH Stern, L Duckett, ED Ponomarev, IA Vorobjev. Circulating microparticles: square the cycle. BMC Cell Biology 14: 23 (2013).

Book