Immune Disease Institute

Overview

The Flow and Imaging Cytometry Resource provides research flow and imaging cytometry services to all investigators in the Prgram in Cellular and Molecular Medicine at Boston Children's Hospital and the local scientific community on a case-by-case basis. With state of the art instrumentation, such as two standard configuration 3-lasers FACSArias located in BL2+-facility and in PCMM core space, and one with 20-parameters 4-lasers FACSAria SORP, the facility offers high speed cell sorting and complex analytical services as well as consulting on design and development of new protocols and methods. In addition, the facility has two 8-color FACSCantos and two dual laser FACSCalibur^TM benchtop analyzers.  In addition, an Imagestream100 imaging cytometer was acquired with a help of NIH Shared Instrumentation Grant.

Our recent publications:

Quantum dots in microscopy and cytometry: immunostaining applications. Barteneva N, Vorobjev I. In: Microscopy Book Series, vol 4, pp 1-16. In Press

Q-dots nanocrystals in intracellular flow: ZAP-70, Ki-67 and five-colors fluorophors panel. Sotnikov I, Jaron S, Konopleva M, O'Brien S, Hillabrant J, Manis J, Brown J, Vorobjev I, Barteneva N. Nanotechnology,Annaheim, 3: 424-427 (2010)

Unique biological properties of catalytic domain directed human anti-CAIX antibodies discovered through phage-display technology. Xu C, Lo A, Yammanuru A, Tallarico AS, Brady K, Murakami A, Barteneva N, Zhu Q, Marasco WA. PLoS One 5(3): e9625 (2010)

Numbers matter: quantitative and dynamic analysis of the formation of a mature immunological synapse using imaging flow cytometry. Ahmed F, Friend S, George TC, Barteneva N, Lieberman J. Journal Immunological Methods, Jun 12 (2009)

Differential stimulation of monocytic cells results in distinct populations of microparticles. Bernimoulin M, Waters EK,, Foy M., Steele BM, Sullivan M., Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD. J Thromb Haemost 7(6): 1019-1028 (2009)

Uncoupling CD21 and CD19 of the B-cell coreceptor. Barrington RA, Schneider TJ, Pitcher LA, Mempel TR, Ma M, Barteneva NS, Carroll MC. PNAS 106(34): 14490-14495 (2009)

Development of multi-color panels with quantum dots-conjugated antibodies for diagnostics of chronic lymphoid leukemia (CLL) by conventional flow cytometer.
Barteneva N, Vorobjev I.Technical Proceedings, Nanotechnology, volume 2 , pp26-28, Houston (2009)

Multifunctional magnetic nanocarriers for image-tagged SiRNA delivery to intact pancreatic islets. Medarova Z, Kumar M, Ng SW, Yang J, Barteneva N, Evgenov NV, Petkova V, Moore A. Transplantation 86(9): 1170-1177(2008)

Chemogenomics and parasitology: small molecules and cell-based assays to study infectious processes. Muskavitch MA, Barteneva N., Gubbels MJ. Comb Chem High Throughput Screen 11(8): 624-646 (2008) Maresz K., Ponomarev ED, Barteneva N, Tan Y, Mann MK, Dittel BN.

IL-13 induces the expression of the alternative activation marker Ym1 in a subset of testicular macrophages. J Reprod Immun 78(2): 140-148 (2008) Yin Yang 1 is a critical regulator of B-cell development Liu H, Schmidt-Supprian M, Shi Y, Hobeika E, Barteneva N et al. Genes Development 21(10): 1179-1189 (2007).